identification of some molecular traits in fluorescent pseudomonads with antifungal activity
نویسندگان
چکیده
we assessed a collection of 47 fluorescent pseudomonas spp., some with known biological control activity against certain soil-borne phytopathogenic fungi such as, macrophomina phaseolina, rhizoctonia solani, phytophthora nicotianae var. parasitica, pythium sp. and fusarium sp. in vitro and the potential to produce known secondary metabolites such as, siderophore, hcn and protease. the results indicated that 66%, 40.42%, 63.82%, 48.94% and 27.65% of strains revealed antagonistic activity against r. solani, m. phaseolina, pythium sp., p. nicotianae and fusarium sp., respectively. among the 47 strains, 76.59%, 97.87% and 17% produced protease, siderophore and hcn, respectively. in this survey, the detection of phld and phla genes was evaluated with a pcr-based assay. we detected phld in strains p-5, p-32, p-47, and phla in strains p-5, p-18, p-34 and p-35. strain cha0 was used as positive control for the detection of both genes. overall, there was no obvious link between inhibition of fungal growth in vitro and production of the antifungal metabolites or existence of phld and phla genes. characterization of fluorescent pseudomonads with potential to produce of 2, 4-diacetylphloroglucinol will further enhance our knowledge of their function in the suppression of root diseases.
منابع مشابه
Identification of Some Molecular Traits in Fluorescent Pseudomonads with Antifungal Activity
We assessed a collection of 47 fluorescent Pseudomonas spp., some with known biological control activity against certain soil-borne phytopathogenic fungi such as, Macrophomina phaseolina, Rhizoctonia solani, Phytophthora nicotianae var. parasitica, Pythium sp. and Fusarium sp. in vitro and the potential to produce known secondary metabolites such as, siderophore, HCN and protease. The resu...
متن کاملDetection of antimicrobial traits in fluorescent pseudomonads and molecular characterization of an antibiotic pyoluteorin
Thirty isolates of fluorescent pseudomonads were obtained from rhizosphere of different crops in Raichur, India. The fluorescent pseudomonad strains were characterized in vitro for biochemical traits, antimicrobial traits, and pyoluteorin antibiotic production. All the isolates that showed fluorescent pigment production under UV light were rod shaped, Gram negative, positive for oxidase, catala...
متن کاملFormulation Development of Some Fluorescent Pseudomonads for Controlling of Meloidogyne incognita on Pistachio
باکتری های محرک رشد گیاه (PGPR) نقش بالقوهای در کنترل نماتد گره ریشه دارند. هدف از این تحقیق، استفاده از ریزوباکترها برای جایگزینی با مواد شیمیایی به منظور کاهش این مشکل در درختان پسته است. در پی مطالعات قبل، برخی از سویهها با توانایی بالا برای محدود کردن فعالیتهای Melodyogyne incognita انتخاب گردید. سه فرمولاسیون مختلف با استفاده از سبوس گندم، پودرسیب و پودر تالک به عنوان حامل و شش استرین م...
متن کاملSiderophores of fluorescent pseudomonads.
Though iron is fourth in abundance in the earth crust it is not readily available to many m icroorga nisms: In the early days of earth life developed in a reductive atm osphere where iron necessary for many physiological processes was abundant in its divalent form. Fe2+-salts are sufficiently w ater soluble to provide an adequate supply of this elem ent to the cells. But as a consequence of th...
متن کاملMolecular identification and in-vitro antifungal susceptibility testing of Candida species isolated from patients with onychomycosis
Background and Purpose: Candida species are the most opportunistic fungi affecting the nails and resulting in onychomycosis. In this study, we identified and evaluated in-vitro susceptibility of the recovered isolates against fluconazole (FLC), voriconazole (VRC), and clotrimazole (CLT) using the Clinical and Laboratory Standards Institute (CLSI) M27-A3 document. Materials and Method...
متن کاملdetection of volatile compounds of medicinal plants with some nano-sorbents using modified or new methodologies and investigation of antioxidant activity of their methanolic extracts
in this work, a novel and fast method for direct analysis of volatile compounds (davc) of medicinal plants has been developed by holding a filament from different parts of a plant in the gc injection port. the extraction and analysis of volatile components of a small amount of plant were carried out in one-step without any sample preparation. after optimization of temperature, extraction time a...
منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of biotechnologyناشر: national institute of genetic engineering and biotechnology
ISSN 1728-3043
دوره 4
شماره 4 2006
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023